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Using Phenolic Compounds and Some Morphological Characters as Distinguishing Factors to Evaluate the Diversity of Perilla Genetic Resources KCI 등재

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한국자원식물학회지 (Korean journal of plant resources)
한국자원식물학회 (The Plant Resources Society Of Korea)
초록

The objectives of this study were to evaluate total phenolic content (TPC) and individual phenolic compounds in leaves of perilla genetic resources, assess whether they could be used as distinguishing factor among germplasms, and evaluate their relationship with some quantitative and qualitative morphological characters. TPC and individual phenolic compounds were determined using Folin-Ciocalteu method and UPLC-PDA system, respectively. Wide variations in TPC (7.99 to 133.70 ㎎GAE/g DE), rosmarinic acid (ND to 21.05 ㎎/g DE), caffeic acid (ND to 1.17 ㎎/g DE), apigenin- 7-O-diglucuronide (ND to 2.21 ㎎ luteolin equivalent (㎎LUE)/g DE), scutellarein-7-O-glucuronide (ND to 5.25 ㎎ LUE/g DE), and apigenin-7-O-glucuronide (ND to 2.81 ㎎ LUE/g DE) were observed. Intensities of green pigment at abaxial and adaxial leaf surfaces were positively correlated with phenolic compounds whereas leaf length and width had negative correlation. Purple pigmented accessions were shorter in leaf length and width but exhibited higher amount of phenolic compounds compared to green pigmented accessions in most cases. Leaf shape was not related with content of phenolic compounds, color of leaves, and length/width of leaves. TPC and individual phenolic compounds along with morphological characters could be useful distinguishing factors for perilla genetic resources.

목차
Abstract
Introduction
Materials and Methods
    Chemicals and Reagents
    Plant Materials
    Sample Preparation
    UPLC-PDA Analysis
    Determination of Total Phenolic Content (TPC)
    Statistical Analysis
Results and Discussion
    Morphological Characters
    Contents of Total and Individual Phenolic Compounds
    Correlations between Phenolic Compounds and MorphologicalCharacters
References
저자
  • Awraris Derbie Assefa(National Agrobiodiversity Center, National Institute of Agricultural Sciences)
  • Yi Jin Jeong(National Agrobiodiversity Center, National Institute of Agricultural Sciences)
  • Ju-hee Rhee(National Agrobiodiversity Center, National Institute of Agricultural Sciences)
  • Ho-Sun Lee(National Agrobiodiversity Center, National Institute of Agricultural Sciences)
  • On-Sook Hur(National Agrobiodiversity Center, National Institute of Agricultural Sciences)
  • Jae-Jong Noh(National Agrobiodiversity Center, National Institute of Agricultural Sciences)
  • Na-Young Ro(National Agrobiodiversity Center, National Institute of Agricultural Sciences)
  • Ae-Jin Hwang(National Agrobiodiversity Center, National Institute of Agricultural Sciences)
  • Jung-Sook Sung(Upland Crop Breeding Division, National Institute of Crop Science, Rural Development Administration)
  • Jae-Eun Lee(National Agrobiodiversity Center, National Institute of Agricultural Sciences) Corresponding author