Many people have often reused their disposable respirator multiple times due to reasons of economy and purchasing inconvenience. However, multiple use of a disposable respirator may lead to an adverse effect of its protection performance. In this study, we aimed to determine changes in the protection performance of disposable respirators related to multiple use and to suggest the appropriate number of times of use. The masks of the brands T and Y were selected in this study, respectively. A mask of each brand was used for testing at the same time and place for two hours per day. The protection performance of each disposable mask was tested after consecutive use on days two, three, and four. The protection performance tests for each used mask following wear on a mannequin were performed in the laboratory using GRIMM’s fine dust monitoring device and TSI’s nebulizer. The concentration of PM10, PM2.5, and PM1.0 inside and outside the mask was measured. The filtration rate was calculated and the results were analyzed using the SAS statistical program. The protection rates of the brandnew masks of T and Y brands (blank test) were 80% and 60%, respectively. After one-day usage, the protection rates of the brand-new T and Y branded masks significantly decreased from 80% to 60% and from 60% to 50%, respectively. Rather, the slight drops of protection rate were observed after two or more days of respirator use. Our findings indicate that the change of protection performance could occur after one-time use. It would not be recommended to use disposable respirators several times in terms of in terms of protecting human health from environmental hazards.