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pp.513-528
Coriandrum sativum L., an annual herbaceous plant of Apiaceae family. The present study evaluated the anti-oxidant activities and anti-inflammatory effects of ethanol extracts of C. sativum. The anti-oxidant activities of C. sativum were measured by total contents of polyphenol, flavonoid, DPPH and ABTS radical scavenging and reducing power activity. And anti-inflammatory effects of C. sativum were measured by LPS-induced RAW 264.7 cells. The results showed that the contents of total polyphenol and flavonoid were 76.03 ± 1.36 mg of gallic acid equivalents/g and 182.23 ± 4.32 mg of rutin equivalents/g at concentration 1 mg/ mL of C. sativum. The DPPH radical scavenging activity was found to be 52.8% at 500 μg/mL. The ABTS radical scavenging activity was shown in 58.3% after exposure to 1,000 μg/mL. Reducing power activity was found to be 66.8% at 2,000 μg/mL. The inhibitory effect of NO production was found to be 65% concentration 500 μg/mL. In the generation quantity of inflammatory cytokines such as TNF-α and IL-1β in cell culture medium, the expression levels of inflammatory proteins in cells were showed decrease with the increase of concentration. Therefore, we suggest that the C. sativum should be a potential source of alternative anti-inflammatory drug with good anti-inflammatory effects.
Ⅱ. 재료 및 방법
1. 실험재료
2. 추출물 제조
3. 총 폴리페놀 함량 측정
4. 총 플라보노이드 함량 측정
5. DPPH radical 소거능 측정
6. ABTS radical 소거능 측정
7. 환원력 측정
8. 세포 배양
9. 세포 생존율
10. Nitric oxide (NO) 생성 억제 활성
11. TNF-α 및 IL-1β 생성 억제 활성
12. Western blotting
13. 통계처리
Ⅲ. 결과 및 고찰
1. 총 폴리페놀 및 플라보노이드 함량
2. DPPH 및 ABTS radical 소거활성
3. 환원력
4. 세포 생존율
5. Nitric Oxide 생성 억제 효과
6. TNF-α 및 IL-1β 생성 억제 효
7. iNOS 및 COX-2의 발현량 억제 효과
8. NF-κB 및 IκBα 인산화 억제 효과
9. ERK 및 P38 인산화 억제 효과
Ⅳ. 적 요
References
