To identify four cyst nematodes (Heterodera schachtii, H. trifolii, H. glycines, H. sojae) that are economically important plant-parasitic nematodes in Korea, restriction fragment length polymorphism (RFLP) by 8 endonucleases (PstI, VspI, AlwI, RsaI, MvaI, EcoRI, Eco72I, Hinf I) was performed based on sequence difference of mitochondrial DNA cytochrome c oxidase subunit I (COI) gene. As a result, species-specific DNA band patterns by RsaI endonuclease were observed in H. schachtii. The specific patterns was in H. trifolii by 3 endonucleases (VspI, AlwI, Hinf I), and was in H. glycines by Hinf I. While, H. sojae was not digested by 4 endonuclease (VspI, AlwI, RsaI, Hinf I). This study showed that four cyst nematodes could be distinguished using RFLP by 4 endonucleases (RsaI, VspI, AlwI, Hinf I) based on the sequence difference of COI gene.

Ⅰ. 서 론
Ⅱ. 재료 및 방법
    1. 씨스트선충 감염 토양 채취 및 씨스트 분리
    2. DNA 추출
    3. 중합효소연쇄반응
    4. 제한효소절편길이다형성 분석
Ⅲ. 결 과
    1. 씨스트선충 4종의 PCR 및 제한효소절편길이다형성
Ⅳ. 고 찰
References

• 고형래(농촌진흥청 국립농업과학원 작물보호과) | Ko Hyoung-Rai
• 강헌일(부산대학교 식물생명과학과) | Kang Heonil
• 박은형(농촌진흥청 국립농업과학원 작물보호과) | Park Eun-Hyoung
• 김은화(농촌진흥청 국립농업과학원 작물보호과) | Kim Eun-Hwa
• 이재국(국립농업과학원 작물보호과 농업연구사) | Lee Jae-Kook Corresponding author