Plant regeneration from stems and leaves was carried out for the growth of biomass, hybrid poplar(Populus alba x P. glandulosa)clone, which has various uses for plant purification. Callus was well induced when stem and leaf tissues were cultured in 1.0 mg/L 2,4-D containing MS medium. Shoot regeneration was best induced by zeatin among growth regulators, and Plant regeneration was more regenerated in leaf-derived callus than in stem-derived callus. The growth of regenerated shoots at high and low concentrations of zeatin was similar to that of the control at low concentrations. As the concentration of growth regulators increased, the growth of regenerated shoots showed a big difference among individuals. Hybrid poplar showed color variation of plant stem in medium containing high concentration of growth regulator. Regenerated individuals were in vitro rooted in MS medium containing 0.5 mg/L IBA after 2 weeks of culture. and transferred to the greenhouse for acclimatization. The study is believed to be widely used for the induction of in vitro variants through organogenesis.

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
    1. Materials and surface sterilization
    2. Callus induction and proliferation
    3. Regeneration of plants from callus
    4. Growth of regenerated plants
    5. Induction of variants and measurement
    6. In vitro rooting and acclimatation
RESULTS AND DISCUSSION
    1. Callus induction
    2. Shoot regeneration from callus
References

• Eun-ji Choi(Division of Environmental Forest Secience(Institute of Agriculture of Life Science), Gyeongsang National University)
• Hak-Gon Kim(Forest Research Department, Gyeongsangnam-do Forest Environment Research Institute)
• Seong-Hyeon Yong(Division of Environmental Forest Secience(Institute of Agriculture of Life Science), Gyeongsang National University)
• Yu-Won Seol(Division of Environmental Forest Secience(Institute of Agriculture of Life Science), Gyeongsang National University)
• Dong-Jin Park(Dept. of seed and seedling management, National Forest Seed and Variety Center)
• Myung-Suk Choi(Division of Environmental Forest Secience(Institute of Agriculture of Life Science), Gyeongsang National University) Corresponding author