Atopic dermatitis (AD) usually develops in patients with an individual or family history of allergic diseases, and is characterized by chronic relapsing inflammation seen specially in childhood, association with IgE hyperproduction and precipitation by environmental factors. and wished to examine closely effect that Polygonum multiflorum isolated PM-E and PM-70M orally adminstration used to atopy dermatitis disease patient get in atopy eruption control experimentally. Atopic dermatitis is a chronically relapsing inflammatory skin disease. Animal models induced by relevant allergens play a very important role in the elucidation of the disease. This study was investigated the anti-allergic effect of PM-E and PM-70M on BMAC induced atopic dermatitis in NC/Nga mice. We summerized as the follow. PM-E and PM-70M significantly reduced the skin number of total cell number, CD4+ and CD11b+/Gr-1 cell compared with positive control and decreased the invasion of CD4+ cell in dorsal skin tissue compared with positive control group by using immunohistochemical staining and chemokine such as eotaxin and CCR3 compared with positive control group. PM-E and PM-70M markedly suppressed invasion and edema of leukocytes and mast cell in dorsal skin. Taken together, these findings suggested that PM-E and PM-70M has an anti-allergic activity and this might be useful for the clinical application to treat allergic diseases such as atopic dermatitis.

Abstract
1. Introduction
2. Experimental Method
    2.1. Reagents and Instruments
    2.2. Statistical processing
3. Results and Discussion
    3.1. Effect of Skin Tissue on Total CellNumber
    3.2. Effect of Fluorescent Flow Cytometryin Back Skin Tissue on the ImmuneCells
    3.3. Effect on skin inflammation
4. Conclusion
References

• Ji-Sun Moon(중원대학교 의료뷰티케어학과) | 문지선
• Eun-Young Choi(알엑스에이피코리아) | 최은영 Corresponding author