Lactic acid bacteria (LAB) were isolated and analysed its fermentation ability in triticale powder at different moisture levels. Furthermore, the antibacterial activity of fermented silage extract against cattle pathogens was also studied. The isolated strains were P. pentosaceus (TC48) and L. brevis (TC50) that confirmed based on biochemical and 16srRNA sequences methods. Extract from LAB fermented silage showed higher antibacterial activity (inhibition zone diameters: 18~24.2 mm) against E. coli P. aeruoginosa, S. aureus and E. Fecalis than the non-inoculated silage extract. TC48 and TC52 strains exhibited high tolerance to artificial gastric, duodenal and intestinal fluids. In summary, lactic acid bacteria mediated fermentation of triticale silage extract showed great antibacterial activity with significant probiotic characteristics might be an effective and safe way to provide new strategies for reducing the incidence of pathogenic bacteria associated diseases in animals.

• Ilavenil Soundharrajan(Grassland and Forage Division, National Institute of Animal Science, RDA)
• Palaniselvam Kuppusamy(Grassland and Forage Division, National Institute of Animal Science, RDA)
• Hyun Jeong Lee(Jangsu Agriculture Technology Center)
• Ki Choon Choi(Grassland and Forage Division, National Institute of Animal Science, RDA)