The aim of this study was to identify strain KCOM 1265 isolated from subgingival plaque at the species level by comparing 16S ribosomal RNA gene (16S rDNA) and genome sequences. The whole genome of strain KCOM 1265 was extracted using the phenol–chloroform extraction method. 16S rDNA was amplified using polymerase chain reaction and sequenced using the dideoxy chain termination method. Pairwise genome comparison was performed using average nucleotide identity (ANI) and genome-to-genome distance (GGD) analyses. The data showed that the percent similarity of 16S rDNA sequence of strain KCOM 1265 was 99.6% as compared with those of Fusobacterium polymorphum ATCC 10953T and Fusobacterium hwasookii KCOM 1249T. The ANI values of strain KCOM 1265 with F. polymorphum ATCC 10953T and F. hwasookii KCOM 1249T were 95.8% and 93.0%, respectively. The GGD values of strain KCOM 1265 with F. polymorphum ATCC 10953T and F. hwasookii KCOM 1249T were 63.9% and 49.6%, respectively. These results indicate that strain KCOM 1265 belongs to F. polymorphum.

Introduction
Materials and Methods
    1. 세균 및 배양
    2. 16S rDNA 염기서열을 이용한 계통분류학적 분석
    3. 세균 지놈 핵산염기서열 결정
    4. 세균 지놈 핵산염기서열 비교분석법에 의한 종-수준에서의동정
Results
Discussion
References

• Soon-Nang Park(Korean Collection for Oral Microbiology, Chosun University/Department of Oral Biochemistry, School of Dentistry, Chosun University)
• Yun Kyong Lim(Korean Collection for Oral Microbiology, Chosun University/Department of Oral Biochemistry, School of Dentistry, Chosun University)
• Joong-Ki Kook(Korean Collection for Oral Microbiology, Chosun University/Department of Oral Biochemistry, School of Dentistry, Chosun University/Institute of Dental Science, Chosun University) Correspondence to