As part of the research program “2018 Rapid screening and identification of freshwater microorganisms using MALDI-TOF/MS library” freshwater samples were collected from a branch of the Nakdong River. Almost 300 antibiotic-resistant bacterial strains were isolated from freshwater samples and subsequently identified by 16S rRNA gene sequencing. Seventeen strains among the isolates shared high 16S rRNA gene sequence similarity (>99.0%) with known species that were not previously recorded in Korea, and each of the isolates also formed a robust phylogenetic clade with the closest species. These species were phylogenetically diverse, belonging to four phyla, seven classes, 10 orders, and 13 genera. At the genus and class level, the previously unrecorded species belonged to Rhodovarius, Xanthobacter, and Shinella of the class Alphaproteobacteria; Ottowia, Simplicispira, and Zoogloea of Betaproteobacteria; Pseudomonas, Acinetobacter, and Shewanella of Gammaproteobacteria; Arcobacter of Epsilonproteobacteria; Sphingobacterium of Sphingobacteriia; Trichococcus of Bacilli; and Leucobacter of Actinobacteria. The previously unrecorded species were further characterized by examining their gram-staining, colony and cell morphology, biochemical properties, and phylogenetic position.

Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
    1. Description of Rhodovarius lipocyclicusKPS-102
    2. Description of Xanthobacter agilis KPS-34
    3. Description of Shinella curvata KPS-58
    4. Description of Shinella yambaruensis KPS-69
    5. Description of Ottowia beijingensis KPS-14
    6. Description of Simplicispira metamorphaKPS-73
    7. Description of Zoogloea oleivorans KPW-80
    8. Description of Pseudomonas litoralis4AnSW4-16
    9. Description of Pseudomonas protegensKPS-50
    10. Description of Acinetobacter celticusKPW-39
    11. Description of Acinetobacter bohemicusKPW-4
    12. Description of Acinetobacter harbinensisKPW-8
    13. Description of Shewanella glacialipiscicolaKPS-21
    14. Description of Arcobacter cibarius 4KPW53
    15. Description of Sphingobacteriumkitahiroshimense KPW-32
    16. Description of Trichococcus flocculiformis4KPW16
    17. Description of Leucobacter komagatae4KPW17
REFERENCES

• Kiwoon Baek(Bioresources Industrialization Support Department, Nakdonggang National Institute of Biological Resources (NNIBR))
• Eui-Jin Kim(Microbial Research Department, Nakdonggang National Institute of Biological Resources (NNIBR))
• Ji-Hye Han(Microbial Research Department, Nakdonggang National Institute of Biological Resources (NNIBR))
• Ahyoung Choi(Strategic Planning Department, Nakdonggang National Institute of Biological Resources (NNIBR)) Corresponding author