Big data analysis methods are useful tools for sorting valuable data and products. Achyranthes Radix root extract (AR) is a well-known herbal medicine in East Asia due to its anti-osteoarthritis, pro-circulatory, and anti-osteoporosis effects. In this stud y, we investigated the liver- and kidney-protective effects of AR by applying big data analysis to traditional medicine. CDDP (cis-diamminedichloridoplatinum) is an effective cancer cell anti-proliferative agent used in the treatment of diverse types of tumors. However, it is clinically limited due to liver and kidney toxicity. The current study was designed to assess the potential protective effects of AR against CDDP-induced hepato-renal toxicity. For this purpose, male Sprague-Dawley (SD) rats were assigned to four groups, each consisting of four animals. Intravenous injection or oral administration of either saline or AR was performed daily for 14 days, whereas CDDP was injected intraperitoneally on day 3 following AR treatment. Serum biochemistry results revealed that CDDP induced clear hepatic and renal damage while the AR treatment groups showed less damage relative to controls. Next, we tested the pharmacokinetics of AR using 20-hydroxyecdysone (20-HE), which is the most abundant component of AR extract. After intravenous administration of AR, the plasma concentration of 20-HE rapidly declined with a terminal half-life (t1/2) of 0.99±0.47 h. The area under the plasma concentration vs. time curve was 24.96±3.5 h*ng/mL. The present study provides valuable tools for further verification studies of the classical herbal literature and its scientific relevance.

Abstract
INTRODUCTION
MATERIALS AND METHODS
    Chemicals
    Big data analysis
    Preparation of AR extract
    Animal studies
    Serum biochemistry analysis
    Pharmacokinetic analysis
    Statistical analysis
RESULTS
    Big data analysis indicates AR shows highest relativeeffective strength score
    AR prevents CDDP-induced liver and kidney damage
    Pharmacokinetic study of AR
DISCUSSION
REFERENCES

• Hyeonsoo Park(Research Center of Stickus corporation)
• Kwang-Youn Kim(Korea Medicine (KM)-Application Center, Korea Institute of Oriental Medicine)
• Byung Wook Lee(Department of Classics and Medical History, College of Korean Medicine, Dongguk University/4Dongje Medical Co., Ltd.)
• Young Woo Kim(School of Korean Medicine, Dongguk University)
• Gon Sup Kim(Research Institute of Life Science, College of Veterinary Medicine, Gyeongsang National University)
• Chang Keun Kang(College of Veterinary Medicine, Gyeongsang National University/Institutes of Animal Medicine, Gyeongsang National University)
• Kwnag Il Park(College of Veterinary Medicine, Gyeongsang National University/Institutes of Animal Medicine, Gyeongsang National University) Corresponding Author.