논문 상세보기
pp.925-933
To develop eco-friendly microbial inoculants, siderophore-producing bacteria were isolated and identified, and their production characteristics and plant growth-promoting abilities were investigated. A strain S21 was isolated from rhizosphere of Korean perilla (Perilla frutescens) and identified as Enterobacter amnigenus by phenotypic properties and 16S rRNA gene sequencing. The highest siderophore production was obtained in a medium containing 0.5% fructose, 0.1% urea, 0.5% K2HPO4 and 0.1% succinic acid. By using this improved medium, siderophore production increased by 2.5 times compared to that of basal medium. The strain S21 showed insoluble phosphate solubilizing, ammonification and antifungal activities, and also produced hydrolytic enzymes (protease and lipase), indoleacetic acid and 1-aminocyclopropane-1-carboxylate deaminase. Our data suggest that E. amnigenus S21 is a potential candidate that can be used as eco-friendly biocontrol agent and biofertilizer.
1. 서 론
2. 재료 및 방법
2.1. Siderophore 생산균주의 분리 및 동정
2.2. Siderophore 생산에 영향을 미치는 배지성분 조사
2.3. Siderophore의 정량분석
2.4. 식물성장 촉진 활성 조사
3. 결과 및 고찰
3.1. 공시균주의 분리 및 동정
3.2. Siderophore 생산을 위한 배지조성 개선
3.3. 공시균주의 식물성장 촉진 활성
4. 결 론
REFERENCES
