In the oral cavity, complex microbial community is shaped by various host and environmental factors. Extensive literature describing the oral microbiome in the context of oral health and disease is available. Advances in DNA sequencing technologies and data analysis have drastically improved the analysis of the oral microbiome. For microbiome study, bacterial 16S ribosomal RNA gene amplification and sequencing is often employed owing to the cost-effective and fast nature of the method. In this review, practical considerations for performing a microbiome study, including experimental design, molecular analysis technology, and general data analysis, will be discussed.

Introduction
Main Body
    1. 16S rRNA 유전자 분석을 통한 미생물 군집 분석
    2. 실험 계획
    3. 샘플링 방법, 보관, DNA 추출
    4. 차세대 유전체 분석 플랫폼
    5. 데이터 분석 방법
Conclusions
References

• Yeuni Yu(Interdisplinary Program of Genomic Science, Pusan National University)
• Seo-young Lee(Interdisplinary Program of Genomic Science, Pusan National University)
• Hee Sam Na(Department of Oral Microbiology, School of Dentistry, Pusan National University) Correspondence to