Resveratrol has been reported to exert anticancer activity via modulation of multiple pathways and genes. In this study, we examined the effect of resveratrol on YD-10B human oral squamous cell carcinoma cells and its molecular mechanisms of action. We found that resveratrol inhibited the proliferation of YD-10B cells in a dose- and timedependent manner. The suppressive effect of resveratrol was accompanied by a reduction in Bmi-1 gene expression. We observed that silencing the Bmi-1 gene by small interfering RNA effectively downregulated the levels of GLUT1 mRNA and protein, which were also repressed by resveratrol. Bmi-1 silencing increased the number of YD-10B cells in S-phase arrest by approximately 2.3-fold compared with the control. In conclusion, the results of the present study demonstrate, for the first time, that resveratrol suppresses Bmi-1-mediated GLUT1 expression in human oral squamous cell carcinoma cells and suggest that the specific molecular targeting of Bmi-1 and/or GLUT1 expression can be combined with a chemotherapeutic strategy to improve the response of oral cancer cells to resveratrol.

Introduction
Materials and Methods
    1. 실험재료
    2. 세포배양
    3. 세포 생존율 측정
    4. RNA 분리 및 역전사 중합효소 연쇄반응(reversetranscription-PCR, RT-PCR)
    5. Western blot analysis
    6. Transient transfection of small interfering (si)RNA
    7. 유세포 분석(flow cytometry analysis)
    8. 면역세포화학 염색법(immunocytochemistry)
    9. 통계처리
Results
    1. 레스베라트롤이 구강암세포의 증식에 미치는 영향
    2. 구강암세포에서 레스베라트롤에 의한 Bmi-1의 발현 변화
    3. Bmi-1 silencing이 구강암세포의 세포주기에 미치는 영향
    4. 구강암세포에서 Bmi-1 silencing에 의한 GLUT1의 발현변화
    5. 구강암세포에서 레스베라트롤에 의한 GLUT1 발현 변화
Discussion
References

• Kyoung-Eun Park(Department of Dental Pharmacology, School of Dentistry, Pusan National University)
• Chang Youp Ok(Department of Dental Pharmacology, School of Dentistry, Pusan National University/BK21 PLUS Project, School of Dentistry, Pusan National University/Periodontal Disease Signaling Network Research Center, School of Dentistry, Pusan National University)
• Hye-Ock Jang(Department of Dental Pharmacology, School of Dentistry, Pusan National University)
• Moon-Kyoung Bae(Periodontal Disease Signaling Network Research Center, School of Dentistry, Pusan National University/Department of Oral Physiology, School of Dentistry, Pusan National University)
• Soo-Kyung Bae(Department of Dental Pharmacology, School of Dentistry, Pusan National University/BK21 PLUS Project, School of Dentistry, Pusan National University/Periodontal Disease Signaling Network Research Center, School of Dentistry, Pusan National University) Correspondence to