Colon cancer is one of the most common malignant tumors, but there are still a few validated biomarkers of colon cancer. Exosome-mediated microRNAs (miRNAs) have been recognized as potential biomarkers in cancers, and miRNAs can regulate a variety of genes. Recently, Fusobacterium nucleatum was discovered in the tissues of human colon cancer patients. Its role in colon cancer was highlighted. F. nucleatum may contribute to the progression of colon cancer through the mechanism of exosome-mediated miRNAs transfer. However, the exosomal miRNAs regulation mechanism by F. nucleatum in colon cancer is not well known. Thus, we performed next-generation sequencing to investigate the overall pattern of exosomal miRNAs expression in the colon cancer cell culture supernatant. We have confirmed the alterations of various exosomal miRNAs. In addition, to investigate the function of exosomal miRNAs, a Kyoto Encyclopedia of Genes and Genomes analysis was performed on the target genes of changed miRNAs. Potential target genes were associated with a variety of signaling pathways, and one of these pathways was related to colorectal cancer. These findings suggested that F. nucleatum can alter exosomal miRNAs released from colorectal cancer cells. Furthermore, exosomal miRNAs altered by F. nucleatum could be potential biomarkers for the diagnosis and therapy of colon cancer.

Introduction
Materials and Methods
    1. 세포 배양과 세균 감염
    2. 세균 배양
    3. 엑소좀 분리와 miRNA 추출
    4. miRNAs 라이브러리 구축 및 NGS 분석
    5. Kyoto Encyclopedia of Genesand Genomes (KEGG)경로 분석
    6. 통계 분석
Results
    1. 대장암 세포 배양 상층액에 포함된 엑소좀 유래 miRNAs발현의 NGS 기반 분석
    2. Heatmap 분석을 통한 엑소좀 miRNAs의 발현 패턴 프로파일링
    3. KEGG 경로와 target 유전자 분석
Discussion
References

• Mi Ra Yu(Department of Oral Pathology, and BK21 PLUS Project, School of Dentistry, Pusan National University/Periodontal Disease Signaling Network Research Center (MRC), School of Dentistry, Pusan National University)
• Hye Jung Kim(Periodontal Disease Signaling Network Research Center (MRC), School of Dentistry, Pusan National University)
• Ji Wan Kang(Interdisciplinary Program of Genomic Science, Pusan National University)
• Yun Hak Kim(Department of Anatomy, School of Medicine, Pusan National University)
• Hae Ryoun Park(Department of Oral Pathology, and BK21 PLUS Project, School of Dentistry, Pusan National University/Periodontal Disease Signaling Network Research Center (MRC), School of Dentistry, Pusan National University) Correspondence to