This study was conducted to evaluate the effect of lactic acid bacteria (LAB) inoculation to domestically-cultivated Italian ryegrass (IRG) on silage fermentation and in vitro ruminal fermentation. There were six treatments based on the LAB inoculants: 1) no addition of LAB (negative control: NC), additions of 2) commercially-available LAB (positive control: PC), 3) Lactobacillus plantarum (LPL), 4) L. paracasei (LPA), 5) L. acidophilus (LA), and 6) L. pentosus (LPT). All treatments were inoculated at a concentration of 106 CFU/g and ensiled for 3, 7, 21, and 42 days in triplicate and analyzed for nutritive values when ensiling was terminated. Day 42 silage from all treatments were also examined for in vitro ruminal fermentation. After 42 days, LAB-inoculated silages had higher (P<0.05) lactic acid concentration compared to the NC. In terms of nutritive values, the silages treated with LPA, LA, and LPT showed higher (P<0.05) crude protein and lower (P<0.05) neutral detergent fiber and acid detergent fiber content compared to the rest of the treatment. In vitro ruminal dry matter degradability was not affected by LAB addition. However, LAB-treated IRG had shown higher (P<0.05) ammonia-N compared with that of the NC. LPA had shown the highest (P<0.05) volatile fatty acid concentration among the LAB examined. In conclusion, the addition of a single strain of LAB appeared to produce a quality IRG silage compared with the NC and the PC. Among the strains examined, LPA seemed to be superior to the others.

ABSTRACT
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
    1. Experimental design
    2. Ensiling process
    3. Sample collection and analyses
    4. In vitro rumen fermentation characteristics
    5. Statistical analysis
Ⅲ. RESULTS AND DISCUSSION
    1. Fermentation and nutritive value of silage
    2. In vitro rumen fermentation characteristics
Ⅳ. CONCLUSION
Ⅴ. ACKNOWLEDGMENTS
Ⅵ. REFERENCES

• Kihwan Lee(Department of Animal Science, Kyungpook National University)
• Tabita Dameria Marbun(Department of Animal Science, Kyungpook National University)
• Suyeon Kim(Department of Animal Science, Kyungpook National University)
• Jaeyong Song(Department of Animal Science, Kyungpook National University)
• Chan Ho Kwon(Department of Animal Science, Kyungpook National University)
• Duhak Yoon(Department of Animal Science, Kyungpook National University)
• Jungsun Kang(Genebiotech Co. Ltd.)
• Chanho Lee(Genebiotech Co. Ltd.)
• Sangbuem Cho(Department of Animal Sciences, Jeonbuk National University)
• Eun Joong Kim(Department of Animal Science, Kyungpook National University) Corresponding author