The aim of this study was to investigate the feasibility of discrimination 12 different cultivar of sorghum × sudangrass hybrid (Sorghum genus) seed through near infrared spectroscopy (NIRS). The amount of samples for develop to the best discriminant equation was 360. Whole samples were applied different three spectra range (visible, NIR and full range) within 680-2500 nm wavelength and the spectrastar 2500 Near near infrared was used to measure spectra. The calibration equation for discriminant analysis was developed partial least square (PLS) regression and discrimination equation (DE) analysis. The PLS discriminant analysis model for three spectra range developed with mathematic pretreatment 1,8,8,1 successfully discriminated 12 different sorghum genus. External validation indicated that all samples were discriminated correctly. The whole discriminant accuracy shown 82 ~ 100 % in NIR full range spectra. The results demonstrated the usefulness of NIRS combined with chemometrics as a rapid method for discrimination of sorghum × sudangrass hybrid cultivar through seed.

ABSTRACT
Ⅰ. 서 론
Ⅱ. 재료 및 방법
    1. 종자 시료 및 스펙트라 수집
    2. 품종판별 검량식 개발 및 검증
Ⅲ. 결과 및 고찰
    1. 수수×수단그라스 교잡종 품종의 초종별 NIRS 스펙트라
    2. 수수×수단그라스 교잡종 종자의 초종 판별 검량식 개발 및 검증
    3. Discrimination equation에 의한 수수×수단그라스 교잡종종자 판별
Ⅳ. 요 약
Ⅴ. 사 사
Ⅵ. REFERENCES

• 이기원(농촌진흥청 국립축산과학원) | Ki-Won Lee (Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration)
• 송요욱(농촌진흥청 국립축산과학원) | Yowook Song (Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration)
• 김지혜(농촌진흥청 국립축산과학원) | Ji Hye Kim (Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration)
• 라하만 아티쿨(농촌진흥청 국립축산과학원) | Md Atikur Rahman (Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration)
• 오미래(농촌진흥청 국립축산과학원) | Mirae Oh (Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration)
• 박형수(농촌진흥청 국립축산과학원) | Hyung Soo Park (Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration) Corresponding author