Hispidin is a secondary metabolite found in numerous medicinal mushrooms that has attracted significant attention, owing to its distinct biological effects, including antioxidant, anti-inflammatory, antitumor, and cytoprotective properties. Experiments are being carried out to study the interaction of detonation nanodiamonds (DNDs) with synthetic and natural hispidin sourced from extracts of Pholiota sp. fungus. The bioluminescence method is used to determine the adsorption/ desorption properties of DNDs toward hispidin. It is found that hispidin forms strong conjugates with DNDs, and the use of various eluents does not result in a significant release of the adsorbed hispidin molecules. DND-bovine serum albumin (BSA) complex, where DNDs serve as a carrier for the protein and the latter acts as a hispidin sorbent, has been developed and applied in hispidin adsorption/desorption tests. The results support the use of the DNDs as a carrier for hispidin in medical applications. They also advocate the application of the DND-BSA complex for isolating the substance from fungal extracts.

Abstract
Introduction
Experimental
    Nanodiamonds
    In vitro luminescence system
    Hispidin sources
    Luminescence measurement
    DND-BSA complex
    Adsorption of hispidin on DNDs and DND-BSAcomplex
Results and discussion
    DND-hispidin conjugates
    DND-BSA-hispidin conjugates
Conclusions
References

• Changkyu Rhee(Nuclear Materials Research Division, Korea Atomic Energy Research Institute (KAERI)) Corresponding Author
• Whungwhoe Kim(Nuclear Materials Research Division, Korea Atomic Energy Research Institute (KAERI))
• Andrey E. Burov(Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”/Federal Research Center for Information and Computational Technologies)
• Alexey P. Puzyr(Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”)
• Vladimir S. Bondar(Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”)