The objective of this study was to produce a nutrient delivery system (NDS) using sialic acid extracted from edible bird’s nest (EBN), which improves brain function in patients with Alzheimer’s disease and Parkinson’s disease, by affinity bead technology (ABT). The inhibitory activity of acetylcholinesterase (AChE) and pyramidal cells in the dentate gyrus of the hippocampus were analyzed to investigate the effect of a sialic acid NDS on Alzheimer’s disease. Also, the effect of a sialic acid NDS on Parkinson’s disease was evaluated by rota-rod test and pole test in an animal model. Among the groups treated with donepezil, EBN, and sialic acid NDS, the AChE activity was the lowest in the sialic acid NDS-treated group. The results of the hippocampus analysis of the rat model confirmed that the sialic acid NDS inhibited amyloid-beta accumulation depending upon the concentration. Also, the sialic acid NDS group showed more improvement in motor deterioration than the1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)- induced group in both the rota-rod test and pole test. Therefore, the sialic acid NDS had an effect of protecting not only Alzheimer’s disease by inhibiting AChE and amyloid-beta accumulation, but Parkinson’s disease by preventing neurotoxicity induced by MPTP.

Abstract
서 론
재료 및 방법
    1. 실험 동물 및 재료
    2. 실험방법
결과 및 고찰
    1. 시알산의 분리정제
    2. 인지능력 개선 효능 분석
    3. 운동기능 개선 효능 평가
요약 및 결론
References

• 김동명(㈜케이제이엠바이오 바이오연구소) | Dong-Myong Kim (Biotechnology Research Institute, KJM Bio Ltd.) Corresponding author
• 정주영(㈜케이제이엠바이오 바이오연구소) | Ju-Yeong Jung (Biotechnology Research Institute, KJM Bio Ltd.)
• 이형곤(㈜케이제이엠바이오 바이오연구소) | Hyung-Kon Lee (Biotechnology Research Institute, KJM Bio Ltd.)
• 권용성(㈜케이제이엠바이오 바이오연구소) | Yong-Seong Kwon (Biotechnology Research Institute, KJM Bio Ltd.)
• 백진홍(㈜김정문알로에 생명과학연구소) | Jin-Hong Baek (R&D Center of Aloe, Kim Jung Moon Aloe Ltd.)
• 한인석(유타대학교 화학공학과) | In Suk Han (Dept. of Chemical Engineering, College of Engineering, The University of Utah)