In this study, chemical information on a total of 20 individual compounds was constructed to identify isoflavones from the previous reports related with used parts(seeds, leaves, stems, pods) and products of soybean(Glycine max L.). Through constructed library and UPLC-DAD-QToF/MS analysis, a total of 19 individual isoflavones including aglycones, glucosides, acetylglucosides and malonylglucosides as major compounds was identified and quantified from 14 selected soybean seeds. Among them, genistein 7-O-(2"-O-apiosyl)glucoside and genistein 7-O-(6"-O-apiosyl)glucoside(ambocin) were identified tentatively as novel compounds in soybean seeds. Besides, among malonylglucosides, glycitein 4'-O-(6''-O-malonyl)glucoside was estimated for the first time. Total isoflavone contents were distributed from 240.21 to 445.21(mg/100 g, dry matter) and 7-O-6''-O-malonylglucosides were composed of 77.8% on total isoflavone as well as genistein derivatives were confirmed as major class. It was considered importantly that the development of isoflavone-rich varieties was necessary to strengthen their effects such as anti-inflammation, anti-cancer and menopause mitigation. The qualitative and quantitative data presented precisely in this study could be help to select and breed isoflavone-rich varieties. Furthermore, their basic isoflavone profile is expected to be applied to estimate the change of isoflavone conjugates on bioavailability after soy food supplements.

Abstract
서 론
재료 및 방법
    1. 실험 재료 및 시약
    2. 이소플라본 추출 및 SPE(Solid phase extraction) 과정
    3. 대두 함유 이소플라본 구조 동정을 위한 라이브러리제작
    4. UPLC-DAD-QToF/MS 분석
    5. 통계 처리
결과 및 고찰
    1. 대두 우수자원 종자 내 개별 이소플라본 성분 분리 및동정
    2. 대두 우수자원 간 이소플라본 조성 및 함량 비교
요약 및 결론
References

• 이소정(국립농업과학원 농식품자원부) | So-Jeong Lee (Dept. of Agro-Food Resources, National Institute of Agricultural Sciences, RDA)
• 김헌웅(국립농업과학원 농식품자원부) | Heon-Woong Kim (Dept. of Agro-Food Resources, National Institute of Agricultural Sciences, RDA)
• 이수지(국립농업과학원 농식품자원부) | Suji Lee (Dept. of Agro-Food Resources, National Institute of Agricultural Sciences, RDA)
• 라혜민(국립농업과학원 농식품자원부) | Hyemin Na (Dept. of Agro-Food Resources, National Institute of Agricultural Sciences, RDA)
• 권령하(국립농업과학원 농식품자원부) | Ryeong Ha Kwon (Dept. of Agro-Food Resources, National Institute of Agricultural Sciences, RDA)
• 김주형(국립농업과학원 농식품자원부) | Ju Hyung Kim (Dept. of Agro-Food Resources, National Institute of Agricultural Sciences, RDA)
• 윤혜명(국립농업과학원 농업유전자원센터) | Hyemyeong Yoon (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA)
• 최유미(국립농업과학원 농업유전자원센터) | Yu-Mi Choi (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA)
• 위치도(국립농업과학원 농식품자원부) | Chi-Do Wee (Dept. of Agro-Food Resources, National Institute of Agricultural Sciences, RDA)
• 유선미(국립농업과학원 농식품자원부) | Seon Mi Yoo (Dept. of Agro-Food Resources, National Institute of Agricultural Sciences, RDA)
• 이상훈(국립농업과학원 농식품자원부) | Sang Hoon Lee (Dept. of Agro-Food Resources, National Institute of Agricultural Sciences, RDA) Corresponding author