In this study, we were performed to elucidate the antioxidant and anticancer activity by leaves extracts from Acer tegmentosum (AT-L). In DPPH, ABTS radical scavenging activity, the AT-L revealed the high scavenging activity. Especially, the AT-L measured the highest ABTS radical scavenging activity, which is higher than ascorbic acid. The types of human cancer cells for evaluating the anticancer activity were colorectal cancer (SW480), prostate cancer (PC-3), breast cancer (MCF-7), pancreatic cancer (AsPC-1), lung cancer (A549) and liver cancer (HepG2). Human cancer cell viability was measured using MTT assay. Treatment of the AT-L decreased the cell viability and induced apoptosis in SW480 cells. These results suggest that extracts of the AT-L can be used as supplementary material for developing the natural antioxidant and anticancer drug for human cancer cells.

Abstract
Introduction
Materials and methods
    Materials
    Sample preparation
    Cell culture and treatment
    Contents of total phenol and flavonoid
    DPPH and ABTS radical scavenging assay
    MTT assay
    Flowcytometric analysis
    Western blot analysis
    Statistical analysis
Results and Discussion
    Antioxidative activities of AT-L
    AT-L reduce the cell viability in human cancer cells
    Flowcytometric analysis of AT-L in SW480 cells
    Inhibitory effect of AT-L on cyclin D1 and β-cateninexpression in SW480 cells
References

• Hyun Ji Eo(Forest Medicinal Resources Research Center, National Institute of Forest Science)
• Gwang Hun Park(Forest Medicinal Resources Research Center, National Institute of Forest Science) Corresponding author
• Da Som Kim(Forest Medicinal Resources Research Center, National Institute of Forest Science)
• Yeongyeong Kang(Forest Medicinal Resources Research Center, National Institute of Forest Science)
• Youngki Park(Forest Medicinal Resources Research Center, National Institute of Forest Science)