Cordyceps has been used in traditional Chinese medicine more than 2000 year ago. In this study, the new Cordyceps militaris was founded and isolated from O-dae mountain in Korea, and was identified its genetic characteristics. The newly isolation strain HB8 was most closet to Cordyceps militaris W141449 (99.82%), Cordyceps militaris JLCY-LI819 (99.82%) and Cordyceps militaris 4642 (99.81%), respectively. the genotypic result was show that train HB8 was belonging to the Cordyceps militaris genus, therefore, Cordycep militaris HB8 proposed with accession number MT835161. This study we find the optimal condition for production of cordycepin from Cordyceps militaris HB8 was 8 ㎎ /g (200 g of pupa, 1 g of KH2PO4, 0.5 g of K2HPO4, 20 g of glucose, 1 g of MgSO4, 0.05 g of vitamin B1, and 1 ㎎ of NAA per liter; light condition 300-700 Lux and day/night was 14 h/10 h) and the optimum condition for the production of adenosine was 2.6 ㎎/g (15 g of skim milk powder, 1 g of KH2PO4, 0.5 g of K2HPO4, 20 g of glucose, 1 g of MgSO4, 0.05 g of vitamin B1, and 1 ㎎ of NAA per liter; light condition 300-700 Lux and day/night was 14 h/10 h).

Abstract
Introduction
Materials and Methods
    DNA extraction, PCR and sequencing
    Parent strain and mycelium culture
    Establishment of optimal culture conditions
    Quantification of adenosine and cordycepin
    Statistical Analysis
Results
    Isolation and Genetic Characterization of Cordycepsmilitaris HB8
    Optimal culture conditions of Cordyceps militaris HB8
Discussion
References

• Jin Feng Li(Department of Oriental Medicinal Biotechnology, Kyung Hee University)
• Van An Hoang(HanbangBio Inc. Kyung Hee University Holdings)
• Jong Chan Ahn(Graduate School of Biotechnology, Kyung Hee University)
• Dong Uk Yang(HanbangBio Inc. Kyung Hee University Holdings)
• Dong Wook Lee(HanbangBio Inc. Kyung Hee University Holdings)
• Deok Chun Yang(Graduate School of Biotechnology, Kyung Hee University) Corresponding author