Large-scale cultivation of Microcystis aeruginosa in different light conditions was conducted for verifying the cell growth in a greenhouse system. Environmental and chemical parameters of the large-scale culture medium were measured for analyzing the interaction between M. aeruginosa and its symbiotic bacteria. During cultivation, a difference in cell growth pattern was observed between control (natural light) and lightlimited groups (reduction of blue, green, and blue/green light, respectively). Comparing the control group, the light reduced groups showed slow and delayed cell growth through the cultivation period. Also, there is differences in the consuming pattern of total nitrogen and total phosphorus which indicated that the possibility of interaction between M. aeruginosa and symbiotic bacteria.

Abstract
INTRODUCTION
MATERIALS AND METHODS
    1. Seed cultivation
    2. Large-scale cultivation of M. aeruginosa sp. infilmed acryl tank in a greenhouse
    3. Flow cytometric analysis for live-cell density
    4. Measuring environmental parameter
    5. Chemical composition analysis
RESULTS AND DISCUSSION
    1. Environmental and chemical parameters
    2. Live cell analysis by flow cytometry
CONCLUSION
REFERENCES

• Taehui Yang(Nakdonggang National Institute of Biological Resource)
• Ja-young Cho(Nakdonggang National Institute of Biological Resource)
• Ha-jin Kang(Nakdonggang National Institute of Biological Resource)
• Chang Soo Lee(Nakdonggang National Institute of Biological Resource) Corresponding author
• Eui-jin Kim(Nakdonggang National Institute of Biological Resource) Corresponding author