Fermented foods of Korean temple are vegetable-based foods and their seasonings are limited to five pungent herbs. The aim of this study was to investigate cell-free supernatants (CFS) of lactic acid bacteria isolated from the fermented foods of Korean temples. Three selected CFS significantly inhibited the biofilm formation of Escherichia coli O157:H7 at 24 h, while one of the selected CFS markedly inhibited the biofilm formation at 72 h. However, only one CFS significantly attenuated the biofilm formation of Staphylococcus aureus at 24 h, but these CFS failed to inhibit the biofilm formation. Moreover, selected CFS effectively inhibited the growth of E. coli O157:H7, whereas the growth of S. aureus was not affected in the presence of CFS. These results suggest that CFS of lactic acid bacteria isolated from fermented foods of Korean temples shows antimicrobial activity agasint Gram-negative E. coli O157:H7, but not at Gramd-positive S. aureus .

Abstract
서 론
재료 및 방법
    균주 및 시약
    유산균 분리 및 확인
    Cell-free supernatants(CFS) 회수
    항균효과 분석
    CFS에 의한 biofilm 형성 억제 분석
    CFS에 의한 병원균의 생장 억제 분석
    통계분석
결과
    분리 유산균의 특성
    CFS에 의한 항균효과
    CFS에 의한 병원균의 biofilm 형성 억제
    CFS에 의한 병원균의 생장 억제
고찰
References

• 임지은(동국대학교 식품생명공학과) | Jieun Yim (Dept. of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University-Seoul)
• 장은영(동국대학교 식품생명공학과) | Eun-Young Chang (Dept. of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University-Seoul)
• 김민영(동국대학교 식품생명공학과) | Min Young Kim (Dept. of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University-Seoul)
• 송다현(동국대학교 식품생명공학과) | Dahyun Song (Dept. of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University-Seoul)
• 강석성(동국대학교 식품생명공학과) | Seok-Seong Kang (Dept. of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University-Seoul) Corresponding author