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Apoptotic activity of demethoxycurcumin in MG-63 human osteosarcoma cells KCI 등재후보
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/405882
Demethoxycurcumin (DMC), which is a curcuminoid found in turmeric, has anti-proliferative effects on cancer cells. However, the effect of DMC on osteosarcoma has not been established. The aim of this study was to examine the effects of DMC on cell growth and apoptosis induction in MG-63 human osteosarcoma cells. This study was investigated using 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromid assay, Live/Dead cell assay, 4’, 6-diamidino-2-phenylindole staining, and immunoblotting in MG-63 cells. DMC induced MG-63 cell death in a dosedependent manner, with an estimated IC50 value of 54.4 μM. DMC treatment resulted in nuclear condensation in MG-63 cells. DMC-induced apoptosis in MG-63 cells was mediated by the expression of Fas and activation of caspase-8, caspase-3, and poly (ADP-ribose) polymerase. Immunoblotting results showed that Bcl-2 and Bcl-xL were downregulated, while Bax and Bad were upregulated by DMC in MG-63 cells. These results indicated that DMC inhibits cell proliferation and induces apoptotic cell death in MG-63 human osteosarcoma cells via the death receptormediated extrinsic apoptotic pathway and mitochondria-mediated intrinsic apoptotic pathway.
Materials and Methods
1. Materials
2. Cell line and cell cultures
3. Cell viability test (MTT assay)
4. Live/Dead cell assay
5. DAPI staining
6. Immunoblotting
7. Data analysis
Results
1. Cytotoxic effect of DMC in MG-63 cells
2. Induction of apoptosis by DMC in MG-63 cells
3. Extrinsic and intrinsic apoptotic pathways inducedby DMC in MG-63 cells
Discussion
References
