Cudraxanthone D (CD) is a natural xanthone compound derived from the root barks of Cudrania tricuspidata . However, the biological functions of CD in human metabolism have been rarely reported until now. Autophagy is the self-degradation process related to cancer cell metastasis. Here, we elucidated the effects of CD on human oral squamous cell carcinoma (OSCC) cells’ metastatic ability. We confirmed that CD effectively decreased the proliferation and viability of SCC25 human OSCC cells in time- and dose-dependent manners. Also, the metastasis phenotype of the SCC25 cell (migration, invasion, and epithelial–mesenchymal transition [EMT]) was inhibited by CD. To further investigate the mechanism by which CD inhibited the metastatic capacity, we detected the relationship between EMT and autophagy in the SCC25 cells. The results revealed that CD inhibited the metastasis of the SCC25 cells by attenuating autophagy. Thus, our findings produced a potential novel agent for the treatment of human OSCC metastasis.

Introduction
Materials and Methods
    1. 세포배양(cell culture)
    2. 세포생존율 측정(cell viability assay)
    3. 세포증식 및 이동능 측정(wound healing assay)
    4. 종양세포 이동 및 전이능(migration & invasion assay)
    5. 면역형광염색(immunofluorescent staining)
    6. 단백질 전기영동(Western blot assay)
    7. 통계 처리(statistical analysis)
Results
    1. Cudraxanthone D가 사람구강편평상피암종세포주의 세포생존율에 미치는 영향
    2. SCC25 세포에 대한 cudraxanthone D의 상피간엽이행억제효과
    3. Resveratrol이 유도하는 SCC25의 자가포식작용에 대한cudraxanthone D의 억제효과가 상피간엽이행에 미치는영향
Discussion
References

• Su-Bin Yu(Department of Dental Hygiene, Gimhae College/Department of Oral Anatomy, School of Dentistry, Pusan National University)
• Tae-Hyun Bang(Department of Oral Anatomy, School of Dentistry, Pusan National University)
• Hae-Mi Kang(Department of Oral Anatomy, School of Dentistry, Pusan National University)
• Bong-Soo Park(Department of Oral Anatomy, School of Dentistry, Pusan National University/Education and Research Team for Life Science on Dentistry, Pusan National University/Institute of Translational Dental Sciences, Pusan National University)
• In-Ryoung Kim(Department of Oral Anatomy, School of Dentistry, Pusan National University/Education and Research Team for Life Science on Dentistry, Pusan National University/Institute of Translational Dental Sciences, Pusan National University) Correspondence to