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Optimization of medium components and incubation time for the production of Paecilomyces tenuipes mycelia in submerged culture KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/405909
pp.1-8
The choice of the culture medium is an important factor for the mass production of mycelia in submerged cultures. The influence of liquid medium on the mycelial dry weight of Paecilomyces tenuipes was investigated in this study. The regression equation is expressed as Y=-1292.94187+17.78612X1+18.92425X2+2.11464X3-0.019375X1X2-0.006276X1X3+0.008177X2X3- 0.070169X1 2-0.292175X2 2-0.008818X3 2, where Y represents the value of the mycelial dry weight (g/L), X1 is the particle size of wood sawdust in liquid medium (mesh), X2 is the concentration of the wood sawdust in liquid medium, and X3 is incubation time (h). The medium was optimized using a response surface methodology, and the optimal medium contained 30 g of wood sawdust (140 mesh), 20 g of glucose, and 10 g/L of peptone. Under these conditions, the mycelial dry weight reached 38.1 g/L (actual value). The culture medium containing wood sawdust is simple and easy to use, highly efficient, and eco-friendly, and its effectiveness in large preparations of P. tenuipes mycelia with low material costs has been demonstrated.
INTRODUCTION
MATERIALS AND METHODS
Maintenance and seed culture
Preparation of liquid medium and components
Determination of mycelial dry weight
Experimental design by Box-Behnken design (BBD) ofresponse surface methodology (RSM) for optimization ofliquid medium composition and incubation time
Determination of cordycepin content by highperformance liquid chromatography
Statistical analysis
RESULTS AND DISCUSSION
Effect of different liquid media on mycelial dry weight
Optimization of screened variables using RSM(BBD) for mycelia growth
REFERENCES
