This study was conducted to observe the effect of fiber digestion enhancement and inhibition factors on fibrolytic bacterial colony growth and fiber digestion in the rumen fermentation environment. In order to promote the fiber digestion, 0.2% NaOH of rice straw was used as a substrate in rumen in vitro fermentation. A 0.1% methylcellulose (MC) was added rumen in vitro culture with untreated rice straw to inhibit fiber digestion. When in vitro culture was performed using untreated rice straw as a substrate, all substrate adherent colonies and rumen suspended colonies of Fibrobacter succinogenes, Ruminococcus flavefaciens and Ruminococcus albus showed an increasing growth as incubation time progressed; however there were significantly more substrate-adherent colonies than rumen fluid floating colonies at all incubation times (p<0.05). Fiber substrate digestibility in in vitro rumen fermentation was significantly higher 0.2% NaOH treated rice straw than untreated substrate (p<0.05). In addition, substrate-adherent colonies of fibrolytic bacteria were significantly more in the NaOH-treated group than in the untreated group for F. succinogenes, R. flavefaciens, and R. albus (p<0.05). When untreated rice straw was added to an in vitro culture, with or without 0.1% MC, to create a rumen environment for inhibiting fiber digestion, substrate digestibility was significantly suppressed compared to that in the untreated group (p<0.05). Additionally, substrate-adherent colonies were significantly fewer in the addition of MC than in the untreated control group for F. succinogenes, R. flavefaciens and R. albus (p<0.05). The results indicate that surface-adherent colonies of bacteria decomposing fiber substrate are far more than rumen fluid floating colonies in the rumen fermentation environment, and the environmental factors of rumen fermentation give same associative effect on the fibrinolytic function of fiber bacteria and its ecological communities.

ABSTRACT1
Ⅰ. 서론
Ⅱ. 재료 및 방법
    1. In vitro 반추위 발효 및 실험 설계
    2. 섬유소 기질 및 소화율 측정
    3. 섬유소분해 박테리아의 생태군락 조사
    5. 통계처리
Ⅲ. 결과 및 고찰
    1. 기질 분해에 따른 섬유소 분해 박테리아의 군락 변화
    2. 기질 분해 촉진에 따른 부착 섬유소 분해 박테리아 군락의 변화
    3. 기질 분해 억제에 따른 부착 섬유소 분해 박테리아 군락의 변화
Ⅳ. 요약
Ⅴ. 참고문헌

• 성하균(상지대학교 동물자원과학과) | Ha Guyn Sung (Department of Animal Science, Sangji University) Corresponding author