A paradigm shift in the government’s energy policy was reflected in its declaration of early closure of old nuclear plants as well as cancellation of plans for the construction of new plants. To this end, unit 1 of Kori Nuclear Power Plant was permanently shut down and is set for decommission. Based on these changes, the off-site transport of spent fuels from nuclear power plants has become a critical issue. The purpose of this study is to develop an optimized method for transportation of spent fuels from Kori Nuclear Power Plant’s units 1, 2, 3, and 4 to an assumed interim storage facility by simulating the scenarios using the Flexsim software, which is widely used in logistics and manufacturing applications. The results of the simulation suggest that the optimized transport methods may contribute to the development of delivery schedule of spent fuels in the near future. Furthermore, these methods can be applied to decommissioning plan of nuclear power plants.