This study was conducted to find an efficient and economical mixing ratio of deodorant and a isolated microorganism to reduce ammonia in livestock manure compost. In this study, a simple experimental device that can compare the degree of odor reduction by connecting the vial containing the odor generating source and the gas detection tube and leaving it to stand was used. This test method cannot accurately measure ammonia concentration according to the characteristics of the detector tube, but it is an easy method to compare various experimental conditions. The microorganism isolated from pig manure, “Enterococcus casseliflavus” was found to have an effect on ammonia reduction. Surfactant (sodium dodecyl sulfate), mineral A, mineral B, sulfur, persimmon leaves, and glycerin used as a deodorant were mixed with E. casseliflavus NO-2-L to find the optimum mixing ratio. When 20% of deodorants and E. casseliflavus NO-2-L were added to the source of odor alone, the ammonia reduction efficiency of NO-2-L was the highest (66.7%) compared with other deodorants. When a mixture of microorganisms and deodorants were used, the ammonia reduction efficiency was the best when the mixing ratio of the deodorant was 20%. When mineral B and sulfur were added, the ammonia concentration reduction efficiency was the highest at 83.3%.