This paper presents the characteristics of gentamicin-loaded into cetyl trimethyl ammonium intercalated montmorillonite (GtM/CTMA/Mt) as a hybrid composite for a slow-released antibacterial delivery systems. The work describes the successful immobilization of gentamicin into the interlayers of surfactant-modified montmorillonite. Physicochemical characterization of the material is carried out by means of X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy. The kinetics of the gentamicin release is investigated by in vitro study and analyzed based on UV–Vis spectrometry. In addition, antibacterial study is performed towards Klebsiella pneumoniae Staphylococcus aureus, Escherichia coli, and Streptococcus pyogenes. The results show that the gentamicin loading into CTMA/ Mt increases the effectiveness of the antibacterial activity, as shown by the higher inhibition zone for all tested bacteria, compared to gentamicin as a positive control. The kinetics study suggests that the gentamicin release obeys the modified Korsmeyer–Peppas model. The physicochemical study and activity test demonstrate the feasibility of the GtM/CTMA/Mt for practical applications.

Abstract
1. Introduction
2. Materials and Method
    2.1 Materials
    2.2 Method
3. Results and Discussion
4. Conclusion
References

• Is Fatimah(Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia) Corresponding author
• Habibi Hidayat(Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia)
• Gani Purwiandono(Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia)
• Saddam Husein(Laboratory of Materials for Energy and Environemnt, Department of Chemistry, Universitas Islam Indonesia)
• Won-Chun Oh(Department of Advanced Materials Science and Engineering, Hanseo University)