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조사료와 농후사료의 급여 순서가 In vitro 반추위 발효성상, 총 가스 발생량과 메탄 발생량에 미치는 영향 KCI 등재
The Effects of Feeding Order of Roughages and Concentrates on in vitro Rumen Fermentation, Total Gas and Methane Production in Hanwoo
pp.119-127
The objective of this study was to evaluate effects of feeding methods on in vitro ruminal fermentation, total gas and methane production in Hanwoo steers. Six Hanwoo steers fitted with rumen cannula (430 ± 21 kg of body weight) were randomly assigned to one of three feeding systems: 1) feeding forage 1 hour after concentrate, 2) feeding concentrate 1 hour after forage, 3) feeding mixed ration. Rumen fluid sampled from each animals was incubated 24 hours with maize or timothy substrates in in vitro. Ruminal pH was increased in feeding method 2 or maize substrate than that of other methods or timothy substrate (P < 0.001). The production of total volatile fatty acid, acetate, propionate, butyrate, and valerate were increased when steers fed diets using feeding method 1 or rumen fluid was incubated with maize substrate (P < 0.001). Increased production of total gas and methane was observed in feeding method 1 and maize substrate compared to those of other methods or timothy substrate (P < 0.001). Due to the inconsistent results between ruminal fermentation and gas production in this study, further research is required to estimate effects of feeding method on enteric fermentation and gas production in in vivo.
Ⅰ. 서론
Ⅱ. 재료 및 방법
1. 공시축과 사양관리
2. 반추위액 채취 및 in vitro 배양
3. 반추위 in vitro 발효성상 분석
4. 통계분석
Ⅲ. 결과 및 고찰
Ⅳ. 요 약
Ⅵ. REFERENCES
