논문 상세보기
Comparison of characteristics of mesenchymal stem cells from bovine KCI 등재
- 언어KOR
- URLhttps://db.koreascholar.com/Article/Detail/408747
pp.63-69
Mesenchymal stem cells (MSCs) are multipotent cells capable of replicating as undifferentiated cells, and thus hold therapeutic implications in field of regenerative medicine and reproductive biotechnology. In the present study, we compared the stem cell properties of bovine ear skin tissue (ESK)- and nasal mucosa (NM)-derived MSCs. Bovine ESK-MSCs and NM-MSCs were successfully isolated by collagenase digestion and maintained proliferative capacity during the 20 consecutive passages. Both ESK-MSCs and NM-MSCs showed similar morphology and expressed common cell surface markers (CD29, CD44, CD90, and CD105). Also, we compared differentiation potentials of bovine ESK-MSCs and NM-MSCs into osteogenic, adipogenic, and chondrogenic lineages through specific staining and quantitative real-time RT-PCR. As results, bovine ESK-MSCs and NM-MSCs could differentiate into mesodermal cell lineages. However, bovine ESK-MSCs and NM-MSCs exhibited difference in expression of differentiation-related specific markers. Specifically, NM-MSCs exhibited increased expression levels of osteocalcin, peroxisome proliferator-activated receptor gamma, and aggrecan compared to ESK-MSCs. Also, ESK-MSCs exhibited increased expression levels of collagen type I, II, and lipoprotein lipase compared to NM-MSCs. We suggest that the nasal mucosa of bovine could be used as a source of bovine MSCs.
서 론
재료 및 방법
소 유래 중간엽줄기세포 분리 및 배양
세포 증식능 분석(cumulative population doubling levelanalysis, CPDL)
정량적 중합효소연쇄반응(quantitative real-time RT-PCR,qRT-PCR)을 이용한 유전자 분석
소 유래 중간엽줄기세포의 분화능 분석
통계학적 분석
결 과
소 유래 중간엽줄기세포의 성상 및 증식능 비교
소 유래 중간엽줄기세포의 세포표면 유전자 발현 비교
소 유래 중간엽줄기세포의 중배엽세포로의 분화능 비교
고 찰
REFERENCES
