In this study, we investigated the antioxidant activity and anticancer effect of Coprinus comatus (C. comatus) extracts. Free radical scavenging capacity of the C. comatus extracts was evaluated in vitro by the DPPH assay. C. comatus exhibited the strong DPPH radical scavenging activity. In vitro tests were also performed to examine the anticancer activity of C. comatus against 293 human normal epithelial kidney cells and various human cancer cell lines including AGS, Hela and HCT-15. C. comatus extracts showed the potent cancer cell-selective growth inhibitory activity against AGS, Hela, and HCT-15 cells, but slightly inhibited 293 normal cell growth. We examined several biological activities such as antitumor effects of C. comatus extracts in vivo. Solid tumors were induced by Sarcoma-180 inoculation in the left groin of BALB/c mice and then C. comatus extracts were oral administered. After 5 weeks, all mice were sacrificed and their tumor weights were measured. C. comatus extracts exhibited the most effective antitumor activity, and the tumor growth rate was inhibited by 55% in comparison with control group. Taken together, these results indicate that C. comatus extracts showed high antioxidant activity and possessed powerful antitumor activity.

Abstract
서 론
재료 및 방법
    먹물버섯의 추출
    항산화 활성 측정
    세포 배양
    Hemocytometer를 이용한 세포 생존율 측정
    MTT assay를 이용한 세포 생존율 측정
    종양세포주의 DNA 단편화
    동물 모델을 이용한 먹물버섯 추출물의 항암 효과 연구
    먹물버섯 추출물 Sarcoma 180 이식 마우스에서 종양증식 억제 효과
    통계 분석
결 과
    먹물버섯 추출물의 항산화 활성
    Trypan blue dye exclusion 법에 의한 암세포주 생존율측정
    MTT assay법 및 위상차 현미경을 이용한 암세포주 생존율 측정
    종양세포주의 DNA 단편화
    동물모델에서 먹물버섯 추출물의 항암 효능
고 찰
REFERENCES

• Hang Hee Cho(Ecobios)
• Sun-Hee Jang(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Jae-Hyeon Cho(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) Corresponding Author