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Spirodelae Herba와 Perilla Frutescens의 혼합물이 두경부암에 미치는 영향 KCI 등재
Anti-cancer Effects of the Mixture of Spirodelae Herba and Perilla Frutescens Extracts on Head and Neck Cancer Cell Line
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/408800
pp.87-98
Spirodelae Herba (SH) and Perilla Frutescens (PF) extracts have been widely used in clinical practice with various disorders for thousands of years. There are some reports regarding the anticancer effects of SH and PF each by each, but their mixture have not been investigated and their mechanisms also have not been clear. The purpose of the present study was to investigate the anticancer mechanisms and their effects of the mixture of SH and PF extracts on head and neck cancer cell line. Head and neck carcinoma KB cells were treated with SH, PF and their mixture. Anticancer effects were investigated by searching cancer cell death pathway; apoptosis and autophagy, which have been regarded to be effective and safe methods. Apoptosis, which is termed a programmed cell death, was observed by TUNNEL assay. Autophagy, which is termed a type II programmed cell death, was observed by acridine orange red staining. Additionally, the protein expressions associated with apoptosis and autophagy were detected for their mechanism by western blots. The mixture of SH and PF extracts induced autophagic and apoptotic cell death simultaneously in cancer cells. And 0.4 mg/ml of the mixture with SH and PF extracts down-regulated the expression of mTOR, however, the expressions of ATG5 and LC3-II, which induced autophagy, up-regulated. The mixture of SH and PF extracts also down-regulated the expressions of Bcl-2, but up-regulate the expressions of PARP-1 cleavage, Caspase-9 cleavage, Caspase-3 cleavage and BAX, which induced apoptosis. Taken together, these results suggested that the mixture of SH and PF extracts induce autophagic and apoptotic cell death simultaneously in head and neck cancer cells and it could be used as an alternative for anti-cancer drugs.
Ⅰ. INTRODUCTION
Ⅱ. Materials and Methods
1. Plant material extraction and samplepreparation
2. Cell viability assay
3. Diff-Quick® staining
4. Acridine orange red staining
5. TUNEL assay
6. Western blot
7. Statistical analysis
Ⅲ. Results
1. Effects of the extracts on cell viability andcell morphology in KB cells.
2. The mode of cell death induced by theextracts of SH, PF and MIX.
3. Autophagic protein expression by SH, PFand MIX
4. Apoptotic protein expression by SH, PF andMIX
Ⅳ. Discussion
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