Precise combinations of probiotics can be useful in dog nutrition, treatment and care. Also, host specificity must be considered in order to increase the effectiveness of probiotics. In this study, Lactobacillus acidophilus HY7032 and Lactobacillus reuteri HY7506 were used, which were isolated from feces of healthy dogs through the verification of pH, bile salt tolerance, and antibacterial activity. In addition, the selected strains were confirmed for activity in immune cells. Briefly, L. acidophilus HY7032 and L. reuteri HY7506 enhanced oxidative burst and phagocytosis of innate immune cell activities in peripheral blood. In addition, beagle were administered vancomycin 50 mg and polymyxin B 100 KU for 7 days, and then 107 CFU of L. acidophilus HY7032 and L. reuteri HY7506 were orally administered for 3 weeks to confirm the effect of improving hair quality. Also, compared with the placebo group, the health improvement effect including stool pattern were confirmed. These results imply that the microflora imbalance caused by antibiotics can be gradually improved through the intake of probiotics. Through this study, it was confirmed that L. acidophilus HY7032 and L. reuteri HY7506 are good probiotics that contribute to the welfare and health of companion animals and have the effect of improving hair quality.

Introduction
Materials and Methods
    pH 및 bile 저항성
    항균 능력 시험
    선천성 면역 방어 활성 확인
    실험동물 처치
    개체 상태에 대한 영향 분석
    피모에 대한 효과 분석
    통계 분석
Result
    L. acidophilus HY7032 및 L. reuteri HY7506의 pH,bile salt 저항성
    L. acidophilus HY7032 및 L. reuteri HY7506의항균능력 평가
    L. acidophilus HY7032 및 L. reuteri HY7506의면역세포 활성효과 분석
    L. acidophilus HY7032 및 L. reuteri HY7506의 체중및 체형에 대한 영향 분석
    L. acidophilus HY7032 및 L. reuteri HY7506의 분변상태에 대한 영향 분석
    L. acidophilus HY7032 및 L. reuteri HY7506의피모에 대한 효과 분석
References

• Hong-Jae Lee(College of Veterinary Medicine, Konkuk University)
• Sang-Won Lee(College of Veterinary Medicine, Konkuk University) Corresponding author