The formation of CaCO3 in microalgal culture is investigated and applied for effective separation of microalgae. The presence of several cationic ions in the culture medium mediates the formation of 3 types of mineral precipitates depending on the concentration of mineral precursors, Ca2+ and CO3 2−, amorphous nano-flakes, rhombohedral calcites, and spherical vaterites. While amorphous phased precipitates are formed for all concentrations of mineral precursor, only calcites are formed for 30 mM solutions of mineral precursor, and mixtures of calcites and vaterites are formed for 50 and 100 mM solutions of mineral precursor. The harvesting efficiency is also dependent on the concentration of the mineral precursor: from 90 % for 10 mM to 99 % for 100 mM after 60 mins’ of gravitational sedimentation. The formation of nano-flakes on the surface of microalgal cells induces the flocculation of microalgae by breaking the stable dispersion. The negatively charged surface of the microalgal cell is compatible not only with nano-flake attachment but also with the growth of calcitic crystals in which microalgal cells are embedded.

Abstract
1. Introduction
2. Methods
    2.1 Strain and cell culture
    2.2. Mineralization of CaCO3 and microalgalharvesting
    2.3. Analytical Methods
3. Results and Discussion
4. Conclusion
References

• Dong Hyun Kim(Graduate School of Energy Science and Technology, Chungnam National University)
• You-Kwan Oh(School of Chemical & Biomolecular Engineering, Pusan National University) Corresponding author
• Kyubock Lee(Graduate School of Energy Science and Technology, Chungnam National University) Corresponding author