To improve the environmental management and resources, in this study, we aimed to investigate the effect of adding oyster shell powder to Hanwoo manure on its characteristics and microbial composition during the storage period. Additives were deposited on top of the manure surface at the rate of 0, 0.5, and 1% of oyster shell powder per 200 g of Hanwoo manure in a plastic container with three replicates; however, untreated manure litter served as the control. Manure characteristics (dry matter, organic matter and crude ash) and microbial composition (lactic acid bacteria, yeast, Bacillus subtilis, Salmonella, and E.coli) were evaluated at day 0, 2, 4, and 8. Manure characteristics exhibited an effect on dry matter, organic matter, and crude ash at day 2 and 8 (p<0.05), and not for day 0 and 4 (p>0.05). With the exception of yeast content at day 4 of storage, lactic acid bacteria, yeast, Bacillus subtilis, Salmonella, and E.coli exhibited no significant differences in all conditions during the storage period. Conclusively, addition of 1% oyster shell powder to Hanwoo manure resulted in slightly better manure characteristics; however, its microbial composition remained unchanged.

Abstract
1. 서 론
2. 재료 및 방법
    2.1. 실험 설계
    2.2. 한우분 특성 분석
    2.3. 미생물 성상 및 발효특성
    2.4. 통계처리
3. 결과 및 고찰
4. 결 론
REFERENCE

• 장홍희(경상국립대학교 축산학과) | Hong Hee Chang (Department of Animal Science, Gyeongsang National University)
• 주영호(경상국립대학교 농업생명과학연구원) | Young-Ho Joo (Institute of Agriculture and Life Science, Gyeongsang National University)
• 서명지(경상국립대학교 축산학과) | Myeong-Ji Seo (Department of Animal Science, Gyeongsang National University)
• 김지윤(경상국립대학교 축산학과) | Ji-Yoon Kim (Department of Animal Science, Gyeongsang National University)
• 이성신(경상국립대학교 농업생명과학연구원) | Seong-Shin Lee (Institute of Agriculture and Life Science, Gyeongsang National University)
• 최정석(경상국립대학교 응용생명과학부(BK21Four)) | Jeong-Seok Choi (Division of Applied Life Science (BK21Four), Gyeongsang National University)
• 정승민(경상국립대학교 응용생명과학부(BK21Four)) | Seung-Min Jeong (Division of Applied Life Science (BK21Four), Gyeongsang National University)
• 노현탁(경상국립대학교 응용생명과학부(BK21Four)) | Hyeon-Tak Noh (Division of Applied Life Science (BK21Four), Gyeongsang National University)
• 김삼철(경상국립대학교 축산학과/경상국립대학교 농업생명과학연구원/경상국립대학교 응용생명과학부(BK21Four)) | Sam-Churl Kim (Department of Animal Science, Gyeongsang National University/Institute of Agriculture and Life Science, Gyeongsang National University/Division of Applied Life Science (BK21Four), Gyeongsang National University) Corresponding author