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Effect of Silicon Application on Growth Response of Alfalfa Seedlings Grown under Aluminum Stress in Pots KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/409914
pp.162-167
Aluminum (Al) stress in acidic pH is known to decrease the growth and productivity of alfalfa. However, not much is known about how the application of silicon (Si) affects the Al stress response in alfalfa. This study was conducted to evaluate the effect of exogenous application of Si on the growth of alfalfa seedlings exposed to Al stress in pots. Alfalfa seedlings grown in pots for 2 weeks were treated either Al stress (pH 4.0, 0.2 mM Al) or Al stress + Si (1 mM) for 5 days, lengths and biomass of shoot and root, and chlorophyll and carotenoid contents in leaf tissues were analyzed respectively. Al stress treatment inhibited shoot and root growth, and decreased fresh and dry weights, and chlorophyll content in leaves, but increased carotenoid content. In contrast, when alfalfa seedlings treated with Al stress combined with Si, delayed growth caused by Al stress of shoot and root of alfalfa seedlings was restored, dry weight was increased and chlorophyll content of leaf tissue was increased, but carotenoid content was decreased. These results suggest that Si has a function of alleviating Al toxicity in alfalfa, of which it exhibits a mitigating effect by a function that overlaps with some of the intracellular functions of carotenoids.
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
1. Growth of alfalfa
2. Al stress treatment and Si application
3. Measurement of plant length and biomass
4. Analysis of chlorophyll and carotenoid
5. Statistical analysis
Ⅲ. RESULTS AND DISCUSSION
Ⅳ. CONCLUSION
Ⅵ. REFERENCES
