Ensiling is the most preferred technology to preserve the silage quality with high nutrients by the presence of lactic acid bacteria. In this study, lactic acid bacteria RJ1 and S22 were used to make the silages from different leguminous plants such as alfalfa, hairy vetch and red clover. Experimental groups were divided into control and LAB inoculated groups. LAB inoculated group; all legumes treated with a mixture of RJ1 and S22 and made an anaerobic condition for 45d. Without the addition of LAB considered the control group. The results showed that the lactic acid content was higher in all silages in response to LAB treatment and acetic acid content was slightly increased except red clover by LAB compared to control silages. A poor silage quality marker butyric acid was reduced all legume silages in response to LAB inoculation than control silages. The organic acid is closely associated with microbial population experimental silages. We noted that higher LAB and lower yeast were found in the silage in response to LAB treatment. The contents of crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF), and total digestible nutrient (TDN) were not altered significantly between control and LAB treated silages. Overall data suggested that the inclusion of additional LAB potentially enhance the silage quality and preserved the nutrients for long period.

ABSTRACT
Ⅰ. INTRODUCTION
Ⅱ. MATERIAL AND METHODS
    1. Cultivation of legumes and place
    2. Lactic acid bacteria (LAB)
    3. Bacterial cultivation and Enumeration
    4. Silage production from leguminous plants
    5. Quantification of organic acids and microbial populationenumeration in ensiled silages
    6. Statistical analysis
Ⅲ. RESULTS
    1. LAB on alfalfa
    2. LAB on hairy vetch
    3. LAB on red clover
Ⅳ. DISCUSSION
Ⅴ. CONCLUSION
Ⅶ. REFERENCES

• Karnan Muthusamy(Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration)
• Soundharrajan Ilavenil(Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration)
• Jeong Sung Jung(Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration)
• Bae Hun Lee(Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration)
• Hyung Soo Park(Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration)
• Ki Choon Choi(Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration) Corresponding author