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Citric Acid Reduces Alkaline Stress-induced Chlorosis, Oxidative Stress, and Photosynthetic Disturbance by Regulating Growth Performance, Antioxidant Activity and ROS Scavenging in Alfalfa KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/409921
pp.210-216
Pollution of agricultural soil by alkaline salts, such as Na2CO3, is a critical and long-lasting problem in cultivable land. The aim of the study was to examine the putative role of citric acid (CA) in alleviating Na2CO3-stress in alfalfa. In this study, Na2CO3 significantly induced leaf chlorosis, inhibited plant growth and photosynthesis related parameters, increased hydrogen peroxide (H2O2) and reduced major antioxidant enzymes (SOD ,CAD, APX) in alfalfa. However, the presence of CA these negative effects of Na2CO3-stress largely recovered. Interestingly, expression of antioxidant and ion transporter genes (Fe-SOD, CAT, APX, DHAR and NHX1) involved in Reactive oxygen species (ROS) homeostasis and oxidative stress tolerance in alfalfa. These findings suggest that CA-mediated Na2CO3 stress alleviation is an ecofriendly approach that would be useful to local farmer for alfalfa and other forage crop cultivation in alkaline soils.
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
1. Plant growth and alkaline treatment
2. Plant growth and physiological parameters
3. Determination of hydrogen peroxide
4. Determination of antioxidant enzyme activity
5. Analysis gene expression
6. Statistical analysis
Ⅲ. RESULTS AND DISCUSSION
1. Alkaline stress-induced chlorosis, photosyntheticdisturbance, and growth inhibition were recoveredby citric acid (CA)
2. Citric acid (CA) induced the activity of major antioxidantenzymes and reduced ROS
3. The expression of antioxidant genes was linked tochanges in corresponding enzyme activities
4. The expression of the genes NHX1 and P5CS1 wasinfluenced by alkaline stress.
Ⅳ. CONCLUSION
Ⅵ. REFERENCES
