This study investigated the preventive effect of Dendropanax morbifera leaf extracts (DMLEs) drew out with water, 30, 50 and 70% ethanol against alcohol-induced hepatotoxicity in vitro and vivo. The 3-(4,5-dimethylthiazol-2-yl)-2,5-dipenyltetrazolium bromide (MTT) assay was performed to assess cytotoxic activity of DMLEs, and the half-maximal inhibitory concentration (IC50) ranged between 1.11 and 2.08 mg/mL on Hep3B cells. In preventing ethanol-induced-hepatotoxicity on Hep3B cells, 30 and 50% ethanol DMLEs were significantly effective compared to the 5% ethanol treatment control. In addition, the 30% ethanol DMLE was orally provided to rats 30 min prior to the administration of ethanol (3 mL/kg body weight). At 1, 3 and 5 h after ethanol treatment, the plasma levels of ethanol and acetaldehyde were determined. The 30% ethanol DMLE effectively decreased the plasma ethanol levels during 5 h but increased the plasma acetaldehyde levels until 3 h and then significantly decreased at 5 h, as compared to the control. These results indicate that the 30% ethanol DMLE possesses a potent preventive effect against ethanol-induced liver toxicity in Sprague-Dawley rats.

Abstract
서 론
재료 및 방법
    세포배양
    황칠나무 잎 추출
    DMLE의 세포독성
    세포에서 에탄올 유도 간독성 예방효과
    실험동물
    DMLE 투여
    혈액채취
    혈중 알코올 및 acetaldehyde 농도 분석
    통계분석
결 과
    DMLEs의 세포독성
    세포에서 에탄올 유도 간 독성 예방 효과
    혈중 에탄올 및 acetaldehyde 농도
고 찰
REFERENCES

• Yongjae Han(Department of Anesthesiology and Pain Medicine, College of Medicine, Inje University)
• Dong-Eon Lee(Department of Biomedical Sciences, College of Medicine and Medical School, Chosun University)
• Cheol-Hee Choi(Department of Biomedical Sciences, College of Medicine and Medical School, Chosun University) Corresponding Author
• Hu-Jang Lee(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) Corresponding Author