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Effects of natural killer cell-derived exosomes in canine mammary tumor model KCI 등재
- 언어KOR
- URLhttps://db.koreascholar.com/Article/Detail/410049
pp.129-137
Natural killer (NK) cells have cytotoxic effects on tumor cells and viral pathogens. NK cell-derived exosomes (NK-exosomes) also express typical NK cell markers and cytotoxic molecules, therefore, exert anti-tumor and immune homeostatic activities. In this study, canine NK-exosomes separated from cytotoxic NK cell supernatant carried specific markers such as CD81, Alix, and Perforin 1. We examined the anti-tumor effects of NK-exosomes in an experimental murine model using the canine mammary carcinoma cells, REM134. REM134 cells were xenografted of mammary fat pad of mice. CD133, Bmi-1, MMP-3, IL-6, TNF-α, and PCNA are useful as a molecular marker for tumorigenesis and metastasis. The treatment of canine NK-exosomes inhibited tumor growth and significantly (p<0.01) downregulated the expression of Bmi-1, MMP-3, IL-6, TNF-α, and PCNA in REM134-treated mice. Also, the expression of CD133, potent cancer stem cell marker, was significantly downregulated in the canine NK-exosomes-treated mice compared with that of the tumor group. Collectively, these results suggested that canine NK-exosomes has a potential capacity for regulation of cancer progression and metastasis against canine mammary carcinoma.
서 론
재료 및 방법
개 유선종양세포 배양
개 자연살해세포 분리 및 유세포 분석
표적세포에 대한 자연살해세포 독성 분석
효소면역측정법을 이용한 interferon-gamma (IFN-γ)분석
개 자연살해세포 유래 엑소좀 수집 및 특이마커 분석
면역부전 마우스를 이용한 개 유선종양 형성 및 모니터링
정량적 중합효소연쇄반응을 이용한 유전자 분석
통계학적 분석
결 과
개 자연살해세포 유래 엑소좀 특성 분석
개 자연살해세포의 표적세포 독성 분석
활성화된 자연살해세포 유래 엑소좀의 항종양 효과
고 찰
REFERENCES
