Subway trains with air cleaners have been newly deployed in the Seoul Metro system. The purpose of this study was to determine differences regarding in-cabin particulate matter with respect to concentrations less than 10 um (PM10) and 2.5 um (PM2.5) through the operation of air cleaners in subway trains. One subway train newly installed with in-cabin air cleaners on Seoul Metro Line number 2 was chosen monitoring in 2020. In-cabin air cleaners were turned-on at both front and back areas while those in the middle area were turned-off while the train was running. In-cabin PM10 and PM2.5 concentrations were measured in each area using a real-time aerosol monitor. Average in-cabin PM10 concentrations were statistically significantly lower (by 15%) in areas with air cleaners turned-on (43.8±12.1 μg/m3) compared to those areas where the air cleaners were turned-off (51.4±15.0 μg/m3). Average incabin PM2.5 concentrations were significantly lower (by 14%) in areas with air cleaners turned on (33.7±12.2 μg/m3) compared to those areas where air cleaners were turned-off (39.2± 14.4 μg/m3). In-cabin PM10 and PM2.5 concentrations ratios were similar regardless of area with air cleaners turned-on or turned-off. The in-cabin PM10 and PM2.5 concentrations were not associated with commute time. Use of air cleaners in subway trains effected reductions in in-cabin PM10 and PM2.5 concentrations.