A mid-story isolation system was proposed for seismic response reduction of high-rise buildings and presented good control performance. Control performance of a mid-story isolation system was enhanced by introducing semi-active control devices into isolation systems. Seismic response reduction capacity of a semi-active mid-story isolation system mainly depends on effect of control algorithm. AI(Artificial Intelligence)-based control algorithm was developed for control of a semi-active mid-story isolation system in this study. For this research, an practical structure of Shiodome Sumitomo building in Japan which has a mid-story isolation system was used as an example structure. An MR (magnetorheological) damper was used to make a semi-active mid-story isolation system in example model. In numerical simulation, seismic response prediction model was generated by one of supervised learning model, i.e. an RNN (Recurrent Neural Network). Deep Q-network (DQN) out of reinforcement learning algorithms was employed to develop control algorithm The numerical simulation results presented that the DQN algorithm can effectively control a semi-active mid-story isolation system resulting in successful reduction of seismic responses.