The objective of this study was to develop a simultaneous method for 8 amino acids including alanine, arginine, glutathione, lysine, ornithine, methionine, threonine and tryptophan in veterinary products using LC-MS/MS. To optimize MS analytical condition of 8 amino acids, each parameter was established by multiple reaction monitoring in positive mode. The chromatogram separation was achieved on a C18 column with mobile phase of 0.1% formic acid in D.W. and 0.1% formic acid in acetonitrile for green technology at a flow rate of 0.4mL/min for 5 min with gradient elution. The developed method was validated for mass accuracy, precision, linearity in veterinary products. Calibration curves were linear over the calibration ranges (0.5 – 10 mg/L) for all the analytes r2>0.99. Average recoveries were 92.96 – 105.61% and relative standard deviations (RSD) were 0.27 – 3.5%. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.04 – 0.83 mg/L and 0.12 – 2.52 mg/L, respectively. All values were corresponded with the criteria ranges requested by CD 2002/657/EC. The application of this method will be helpful in quality control analysis of amino acids in veterinary products.

서 론
재료 및 방법
시 약
표준품 및 시험물질 조제
LC-MS/MS 분석조건
분석법 검증
결 과
LC-MS/MS 분석 조건 확립
분석법 검증
고 찰

• HyunYoung Chae(Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea)
• Ok-Mi Jeong(Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea)
• Moon Her(Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea)
• JeongWoo Kang(Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea) Corresponding author