Forage crop management is severely challenged by global warming-induced climate changes representing diverse a/biotic stresses. Thus, screening of valuable genetic resources would be applied to develop stress-tolerant forage crops. We isolated two NAC (NAM, ATAF1, ATAF2, CUC2) transcription factors (ANAC032 and ANAC083) transcriptionally activated by multi-abiotic stresses (salt, drought, and cold stresses) from Arabidopsis by microarray analysis. The NAC family is one of the most prominent transcription factor families in plants and functions in various biological processes. The enhanced expressions of two ANACs by multi-abiotic stresses were validated by quantitative RT-PCR analysis. We also confirmed that both ANACs were localized in the nucleus, suggesting that ANAC032 and ANAC083 act as transcription factors to regulate the expression of downstream target genes. Promoter activities of ANAC032 and ANAC083 through histochemical GUS staining again suggested that various abiotic stresses strongly drive both ANACs expressions. Our data suggest that ANAC032 and ANAC083 would be valuable genetic candidates for breeding multi-abiotic stress-tolerant forage crops via the genetic modification of a single gene.

ABSTRACT
Ⅰ. Introduction
Ⅱ. Materials and Methods
    1. Plant materials and stress treatments
    2. Northern blot analysis
    3. Semi-quantitative RT-PCR analysis
    4. ANAC032- and ANAC083-GUS promoter constructs
    5. Histochemical GUS assay
    6. In vivo targeting of GFP fusion constructs
Ⅲ. RESULTS AND DISCUSSION
    1. Isolation of multi-abiotic stresses responsible genesby microarray analysis.
    2. The transcriptional pattern of ANAC032 and ANAC083.
    3. Subcellular localization of ANAC032 and ANAC083.
    4. Promoter activity of ANAC032 and ANAC083.
Ⅳ. CONCLUSION
Ⅵ. REFERENCES

• Netty Ermawati(Department of Agricultural Production and Central Laboratory for Biosciences, State Polytechnic of Jember)
• Jong Chan Hong(Division of Life Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
• Daeyoung Son(Department of Plant Medicine, Institute of Agriculture and Life Science, Gyeongsang National University) Corresponding author
• Joon-Yung Cha(Research Institute of Life Sciences, Gyeongsang National University) Corresponding author