The present study was aimed to estimate the effect of ensiling period and bacterial inoculants on chemical compositions and fermentation characteristics on rye silage harvested at delayed stage. Rye (Secale cereale L.) was harvested after 20 days of heading stage (29.4% dry matter, DM). The harvested rye forage was applied with different inoculants following: applications of distilled water (CON), Lactobacillus brevis (LBB), Leuconostoc holzapfelii (LCH), or mixture of LBB and LCH at 1:1 ratio (MIX). Each forage was ensiled into 20 L mini bucket silo (5 kg) for 50 (E50D) and 100 (E100D) days in triplicates. The E50D silages had higher in vitro digestibilities of DM (IVDMD, p<0.001) and neutral detergent fiber (IVNDFD, p=0.013), and lactate (p=0.009), and acetate (p=0.011) than those of E100D, but lower pH, lactic acid bacteria (LAB), and yeast. By inoculant application, LCH had highest IVDMD and IVNDFD (p<0.05), while MIX had highest lactate and lowest pH (p<0.05). The CON and LCH in E50D had highest LAB and yeast (p<0.05), whereas LBB in E100D had lowest (p<0.05). Therefore, this study concluded that LCH application improved the nutrient digesbility (IVDMD and IVNDFD) of lignified rye silage, and longer ensiling period for 100 days enhanced the fermentation characteristics of silage compared to ensiling for 50 days.

ABSTRACT
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
    1. Silage production
    2. Chemical compositions and in vitro digestibility
    3. Fermentation characteristics
    4. Microbial enumerations
    5. Statistical analysis
Ⅲ. RESULTS
    1. Chemical compositions and in vitro digestibility
    2. Fermentation characteristics
    3. Microbial counts
Ⅳ. DISCUSSION
Ⅵ. REFERENCES

• Seong Shin Lee(Institute of Agriculture & Life Science, Gyeongsang National University)
• Young Ho Joo(Institute of Agriculture & Life Science, Gyeongsang National University)
• Jeong Seok Choi(Division of Applied Life Science (BK21Plus), Gyeongsang National University)
• Seung Min Jeong(Division of Applied Life Science (BK21Plus), Gyeongsang National University)
• Dimas Hand Vidya Paradhipta(Faculty of Animal Science, Universitas Gadjah Mada)
• Hyeon Tak Noh(Division of Applied Life Science (BK21Plus), Gyeongsang National University)
• Ouk Kyu Han(Department of Crop Science, Korea National College of Agriculture and Fisheries)
• Sam Churl Kim(Institute of Agriculture & Life Science, Gyeongsang National University/Division of Applied Life Science (BK21Plus), Gyeongsang National University) Corresponding author