Unarmored and thin-walled dinoflagellates were collected from brackish and coastal waters of Korea from August 2019 to August 2021. A total of 10 species belonging to orders Sussiales and Gymnodiniales were isolated and established as clonal cultures. Of them, five species (Biecheleria brevisulcata, Lepidodinium chlorophorum, Karlodinium decipiens, Kirithra asteri, and Wangodinium sinense) are newly recorded in Korea and examined using a light microscope (LM) and a scanning electron microscope (SEM). Their molecular phylogeny was inferred from LSU rDNA sequences. Here, we present taxonomic information, morphological features, and molecular phylogenetic positions of these unrecorded dinoflagellate species.