As a herbal supplement, Dioscorea batatas Decne (DBD) presents potent antioxidant activity and diverse health benefits. In the present study, functions of a 30 kDa glycoprotein isolated from DBD (hereafter, DBD glycoprotein) in the regulation of feed efficiency and fecal malodor in mice were explored. DBD glycoprotein produced protective effect against cytotoxicity induced by the ecotoxicological endocrine-disrupting substance bisphenol A in gastrointestinal epithelial HT-29 cells. To investigate its potential roles in the regulation of feed efficiency and fecal malodor, mice were administered an oral injection of DBD glycoprotein for 2 weeks. Compared with the control values, the weight of internal organs (liver, heart, kidney, and spleen) and levels of glutamate pyruvate transaminase, glutamate oxaloacetate transaminase, and lactic dehydrogenase were not significantly changed during DBD glycoprotein administration for 2 weeks. Interestingly, DBD glycoprotein improved feed efficiency and reduced hydrogen sulfide concentration without altering the ammonia level in mouse feces. Collectively, these results indicate that DBD glycoprotein is a functional agent that exerts gastrointestinal protective effects against ecotoxicological substances, improves feed efficiency, and reduces fecal malodor.

1. 서 론
2. 재료 및 방법
    2.1. 시약
    2.2. 마 당단백질 분리 및 정제
    2.3. 세포배양
    2.4. MTT 분석
    2.5. 식이 효율 및 조직세포 독성 측정
    2.6. GPT, GOT 및 LDH 측정
    2.7. 황화수소 및 암모니아 농도 측정
    2.8. 통계처리
3. 결과 및 고찰
    3.1. 장 상피 세포에서 마 당단백질의 환경유해물질에대한 세포보호효과
    3.2. 마 당단백질의 마우스 장기 및 조직세포 독성에 미치는 영향
    3.3. 마 당단백질이 마우스 식이 효율 및 증체량에 미치는 영향
    3.4. 마 당단백질이 마우스 분뇨 악취저감에 미치는 효과
4. 결 론
REFERENCE

• 김도완(대구한의대학교 제약공학과) | Do-Wan Kim (Department of Pharmaceutical Engineering, Daegu Haany University, Gyeongsan 38610, Korea)
• 박문기(대구한의대학교 제약공학과) | Moon-Ki Park (Department of Pharmaceutical Engineering, Daegu Haany University, Gyeongsan 38610, Korea)
• 김태훈((주)푸디웜) | Tae Hoon Kim (FoodyWorm Inc., Choenju 28118, Korea)
• 이세중(대구한의대학교 제약공학과) | Sei-Jung Lee (Department of Pharmaceutical Engineering, Daegu Haany University, Gyeongsan 38610, Korea) Corresponding autho