본 연구는 조사료 원료인 마늘 껍질의 사료적 가치와 메탄 발생량을 조사하기 위해 in vitro 발효 실험을 수행하였다. Garlic husk를 제외하고 국내에서 널리 사용되는 4가지 조사료 원료(Oat hay, Annual ryegrass, Timothy, Tall fescue)를 실험의 처리구로 구성하였다. In vitro 48 시간 발효 후 건물 소화율, 섬유소 소화율, 암모니아태 질소, 휘발성 지방산, pH, 메탄 발생량을 평가하였다. 가스 발생량은 3, 6, 9, 12, 24, 36 및 48 시간에 각각 측정되었다. 최종 가스 발생량에서 Oat hay가 유의적으로 가장 높았다(p<0.01). Garlic husk는 처리구 중 가장 높은 메탄 발생량을 나타내었다(p<0.01). Garlic husk의 건물 소화율은 Oat hay보다 유의적으로 낮았지만 Annual ryegrass, Timothy, Tall fescue보다 높았다(p<0.01). 섬유소 소화율은 Annual ryegrass에서 가장 낮았다(p<0.01). 총 휘발성 지방산 생성량은 Oat hay에서 가장 높았고(p<0.01), Garlic husk, Timothy, Tall fescue는 유사하였다. 본 연구의 in vitro 발효 성상과 메탄 발생량을 고려할 때, Garlic husk는 국내 주요 조사료를 대체할 영양적 가치를 포함하고 있으나 메탄 저감 효과는 미미할 것으로 추정된다.

An in vitro rumen fermentation study was conducted to investigate the nutritional value and methane production when Garlic husk was used as a forage source. Except for Garlic husk, four types of forage sources widely used in Korea (Oat hay, Annual ryegrass, Timothy, and Tall fescue) were constituted as treatment groups in the experiment. Dry matter digestibility, neutral detergent fiber digestibility, ammonia nitrogen, volatile fatty acids, pH and methane production were evaluated after in vitro fermentation for 48 h. Gas production was measured at 3, 6, 9, 12, 24, 36, and 48 h incubations. Oat hay was significantly highest in final gas production (p<0.01). Garlic husk had the highest methane production among treatments (p<0.01). The dry matter digestibility in Garlic husk was significantly lower than that in Oat hay but, higher than that in Annual ryegrass, Annual ryegrass had the lowest neutral detergent fiber digestibility among treatments (p<0.01). Total volatile fatty acids production was the highest in Oat hay (p<0.01), and Garlic husk, Timothy, and Tall fescue were similar. Considering the results of in vitro rumen fermentation and methane production in this study, Garlic husk contains the nutritional value to replace major domestic forage, although it is estimated that the methane reduction effect is insignificant.

초록
Abstract
서론
재료 및 방법
    1. 공시재료 및 공시동물
    2. 영양소 성분분석
    3. In vitro 발효 평가
    4. Gas parameter
    5. 통계 분석
결과 및 고찰
References

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